Journal: Cell reports

Article Title: TRMT1L-catalyzed m 2 2 G27 on tyrosine tRNA is required for efficient mRNA translation and cell survival under oxidative stress

doi: 10.1016/j.celrep.2024.115167

Figure Lengend Snippet:

Article Snippet: The RNA samples were brought to a final concentration of 20 mM HEPES-KOH (pH 7.5) and loaded onto a Bio-Rad EconoFit Macro-Prep DEAE columns pre-equilibrated sequentially with the following buffers: 6 column volumes (CV) of low salt buffer (200 mM NaCl, 8 mM MgCl 2 , 20 mM HEPES-KOH pH 7.5), followed by 15 CV of high salt buffer (450 mM NaCl, 16 mM MgCl 2 , 20 mM HEPES-KOH pH 7.5), then 15 CV of low salt buffer, and finally, 15 CV of binding buffer (100 mM NaCl, 20 mM HEPES-KOH pH 7.5).

Techniques: Recombinant, Membrane, Reverse Transcription, Protease Inhibitor, Magnetic Beads, Staining, Mutagenesis, Silver Staining, BIA-KA, RNA Sequencing, Plasmid Preparation, Software